Exact(6)
In addition, HPV-positivity was assessed by low-stringency Southern blot analysis of PCR products with a cocktail probe of HPV-specific DNA fragments.
High-risk human papillomavirus testing was performed by GP5+/6+ PCR-EIA, using a cocktail probe of 14 hrHPV types, and independent of cytology results.
The effects of TAE on five CYPs activity and mRNA levels were quantitated by cocktail probe drugs using a rapid chromatography/tandem mass spectrometry (LC-MS/MS) method and reverse transcription-polymerase chain reaction (RT-PCR), respectively.
In addition, EIA-negative samples were tested by low-stringency Southern blot analysis of PCR products with a cocktail probe of HPV-specific DNA fragments to assess whether HPV types were present that were not represented in EIA oligoprobe cocktails.
HPV positivity was assessed by Southern blot hybridization of GP5+/GP6+ PCR products with a cocktail probe of specific [α-P]dCTP labelled DNA fragments from cloned DNA of HPV6, 11, 16, 18, 31 and 33 under low stringent conditions (van den Brule et al, 1990; de Roda Husman et al, 1995a, b).
All hrHPV samples were tested with the clinically validated GP5+/6+ PCR with enzyme-immunoassay read-out using a cocktail probe for 14 hrHPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68), according to established protocols (van den Brule et al, 2002; Snijders et al, 2005).
Similar(54)
Cocktail, cocktail, cocktail, cocktail......
For chromosome 1-, 5-, 8- and 12-mband cocktail probes, an additional labelling was carried out using biotin, which is detected by Streptavidin-Cy™5 (B-tect).
HPV positive samples were first subjected to HPV group-specific analysis using cocktail probes for HR and LR HPVs (Jacobs et al, 1997, 2000).
Briefly, HPV-positive samples were subjected to EIA HPV group-specific analysis using cocktail probes for high- and low-risk HPVs (Jacobs et al, 1997).
For the EIA, HPV-positive samples were first subjected to a group-specific analysis using cocktail probes for high-risk (HR) and low-risk (LR) HPVs before individual typing was performed.
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