Cocktail, cocktail, cocktail, cocktail......
Further experiments showed that giving the bacterial cocktail to previously germ-free mice reduced inflammation in the airways of their pups.
For intracellular staining of iNOS, Cell Stimulation Cocktail (a cocktail of phorbol 12-myristate 13-acetate, ionomycin, brefeldin A and monensin from eBioscience) was added and cultured for the last 5 hours before flow cytometric analysis as previously described [ 25].
Long-term liquid culture of primary cells was performed in the presence of a cytokine cocktail as previously described [15].
Following centrifugation (600gmax), supernatant was carefully removed and 20 µL of previously titrated surface marker cocktail was added (CD4-APC-Cy7, CD8-PerCP; BDPharmingen).
The family of a convicted murder who was put to death in Ohio using a previously untested cocktail of drugs has said his prolonged execution was unconstitutional and should not be used again in a federal lawsuit.
We assessed the relevance of protease activity for cooperative invasion by incubating engrafted embryos with a previously described cocktail of protease inhibitors (Sahai and Marshall, 2003; Wolf et al., 2003).
Like other states, Missouri has previously used a cocktail of three-drugs to conduct executions.
Upon being thawed, 5 µl of purified proteasomes were incubated with solubilized ZP protein from 100 oocytes either in the presence of the vehicles or the inhibitor cocktail as previously stated.
This medium consisted of the same basal medium as used for the CD34 expansion step supplemented with 10% HS, the low-dose cytokine cocktail (as previously mentioned) and a new high-dose cytokine cocktail consisting of 20 ng/ml IL-7, 22 ng/ml SCF, 1000 U/ml IL-2 (Proleukin®; Chiron, München, Germany) and 20 ng/ml IL-15 (CellGenix).
