Sentence examples for cocktail of mouse from inspiring English sources

C2 myoblasts were transfected with GFP cDNA mixed with a cocktail of mouse cortactin-specific small interfering RNAs (siRNAs) or siRNAs against unrelated proteins; cells were then allowed to differentiate for 4 d before they were treated overnight with agrin.

C: cocktail of mouse anti-TH (1 400) and rabbit anti-SynI (1 1000) in PBS for 48 h at 4°C.

Briefly, tissue sections were incubated with a cocktail of mouse monoclonal and rabbit polyclonal antibodies, followed by incubation with a cocktail of biotin-conjugated anti-rabbit Ab and HRP conjugated anti mouse antibody (DAKO, CA) for 1 hr, and incubated with alkaline phosphatase-conjugated streptavidin for 30 min (Vector Laboratories, CA).

Slides were incubated with a cocktail of mouse anti-pan-Mena (1 1000 dilution, BD Biosciences, San Jose, CA, U.S.A., catalog number 610693) mixed with rabbit anti-Mena11a (1 500 dilution of 1 mg/ml stock, generated in the lab of FG) in the blocking buffer overnight at 4 °C.

Sections were then incubated overnight in primary antibody (a cocktail of mouse monoclonal anti-neurofilament 2H3 and anti-SV2 from the Developmental Studies Hybridoma Bank), washed the following day and incubated overnight with Alexa-Fluor-488 conjugated anti-mouse IgG1 and Alexa-Fluor-594 conjugated α-bungarotoxin (BTX, Invitrogen).

Slides were incubated with a cocktail of mouse anti-pan-Mena (1 1,000 dilution; BD Biosciences, San Jose, CA, USA, catalog number 610693) mixed with rabbit anti-Mena11a (1 500 dilution of 1 mg/ml stock; generated in the laboratory of FBG) in the blocking buffer overnight at 4°C.

For immuno-histochemical staining, monoclonal mouse anti-human CD31 (1∶25, DAKO clone JC70A, Carpinteria, CA) antibodies, and a cocktail of mouse-anti-cytokeratin, or MAK6 antibodies (predilute, Invitrogen, Carlsbad, CA) were used.

It has been reported that only high concentrations of anti-HA specific cocktails of mouse monoclonal antibodies transferred repeatedly resulted in full virus clearance in immune deficient mice in an influenza treatment model [21], [22].

Nitrocellulose blots were reacted with Roche's anti-GFP cocktail of two mouse monoclonal antibodies (clones 7.1 and 13.1) (1/5000) in combination with peroxidase-coupled sheep anti-mouse IgG (Amersham, 1/4000) as secondary antibody.

After preblocking with 5% FBS, 0.2% bovine serum albumen (BSA)/PBST for 1 hour at room temperature, the embryos were incubated overnight at 4°C in a cocktail of QH1 mouse antibody (1∶1000 dilution) (Developmental Studies Hybridoma Bank) and anti EGFP rabbit antibody (1∶1000 dilution) (Clontech) in 5% FBS, 0.2% BSA/PBST, followed by washing six times for 30 min each in PBST.

Subsequently, the embryos were blocked again with 5% FBS, 0.2% BSA/PBST for 30 min at room temperature, and incubated overnight at 4°C with a cocktail of anti mouse IgG-Alexa 688 goat antibody (1∶1000 dilution) (Clontech) and anti rabbit IgG-FITC donkey antibody (1∶500 dilution) (Abcam), and Hoechst (1∶10,000) (Invitrogen) followed by washing six times for 30 min each in PBST.