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A cocktail of appropriate antibody combinations of up to 8 unique colors from the following was used for staining cells: CD28, CD16 (Beckman Coulter, Miami, FL), CD8, CD14, CD16, CD19, CD45RA (Caltag Labs, Burlingame, CA), CD45RA, CD45RO, CD57, CCR7, CD27 (BD Pharmingen, San Diego, CA), CD4, CD45RA (BD Immunocytometry Systems, San Jose, CA) and CD45RA (Beckman Coulter, CA).
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Cells were washed and then stained for 30 minutes with the appropriate cocktail of the following antibodies from BD Biosciences: CD4-PerCP-Cy5.5, CD8-APC, Vβ8 FITC, Vα2-PE, CD69-PerCP-Cy5.5, CD44-FITC, CD25-PE, CD62L-APC and TCRCα-APC.
The primer extension reaction was performed in a volume of 10 µl, containing 3 µl of PCR product, 2.5 µl of SNaPshot Multiplex Ready Reaction mix, and the appropriate cocktail of PAGE-purified extension primers (IDT Supporting Information Tablee S6B).
Cell suspensions were blocked with 2.4G2, anti-FCγR washed, resuspended in FACS buffer (PBS, 2 mM EDTA, 2% FBS, 0.2% NaN3) containing the appropriate cocktail of antibodies and incubated on ice for 20 min. For intracellular cytokines detection, Fix and Perm® kit (Invitrogen) was used according to manufacturer's instructions.
The solution then provides insight into the appropriate mix or "cocktail" of anti-viral agents that can be designed to deal with the virus' ability to mutate.
The choice of appropriate substrate is important for the successful production of fungal enzymes, as complex feedstocks are known to induce expression of complex lignocellulolytic enzyme cocktails to ensure complete substrate hydrolysis [ 10].
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In addition to appropriate hydrolysis conditions, a fine cocktail of cellulolytic enzymes comprising cellulases, β-glucosidases, xylanases is required to obtain sugar monomers from SCAA-SB [ 3].
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