Cocktail, cocktail, cocktail, cocktail......
Because these drinks are lighter than traditional cocktails, dilution with sparkling water tends to make them taste like something to be poured over ice rather than into a more elegant cocktail glass.
Immunohistochemical staining with CK5/CK14/p63 antibody cocktail (XM26, dilution 1 400, Novocastra, Newcastle upon Tyne, UK; LL002, dilution 1 400, Novocastra; 4A4+Y4A3, dilution 1 1,500, Neomarkers, Fremont, CA, USA, respectively) and with p53 antibody (DO-7, dilution 1 500, Novocastra) was performed as described previously [ 12, 22].
To prepare whole cell lysates, cell pellets were washed in PBS and lysed using standard RIPA buffer supplemented with a protease inhibitor cocktail (1∶50 dilution of lysis buffer).
For the purified hamster anti-mouse TCR γδ monoclonal antibody a secondary biotin anti-hamster cocktail (1∶100 dilution; BD Pharmingen) was used.
The nuclear pellet was resuspended in 30 µl nuclear extraction buffer containing 0.4 M KCl, 5 mM 2-mercaptoethanol and protease inhibitor cocktail (1∶100 dilution, Sigma) in STM buffer, and incubated on ice for 30 min with intermittent shaking and then centrifuged at 14,000×g for 20 min at 4°C.
or HER-2 (clone CB11/TAB250 (cocktail), 1 300 dilution, Novocastra, Newcastle upon Tyne, U.K. and Zymed, South San Francisco, CA, U.S.A).
Subconfluent LM 8 cells were treated for 3 days with TGZ at the indicated concentrations, harvested in 0.3 ml of solution A containing a protease inhibitor cocktail (1 100 dilution; Calbiochem-Novabiochem Co., La Jolla, CA), sonicated briefly at 0°C, and centrifuged.
Rat hearts were homogenized in a lysis buffer containing 20 mmol/L Tris HCl (pH 8.0), 100 mmol/L NaCl, 1 mmol/L EDTA, 10%NP-40NP-40ol/vol) and a protease inhibitor cocktail (1 100 dilution, Sigma).
Acutely dissected DRGs were lysed in ice-old FA-M2 Lysis Buffer (50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, pH 7.5) supplemented with a protease inhibitor cocktail (Sigma 1 100 dilution) by sonication.
