Sentence examples for cocktail cell from inspiring English sources

Cells and total proteins from the cells were collected using cell lysis buffer supplemented with protease inhibitor cocktail (Cell Signaling #9803, Roche complete mini #1836153) after 30 minutes, 1 hour, and 2 hours of insulin stimulation for the low-dose T pre-exposed cells, and after 10, 20 and 30 min of insulin stimulation for the high-dose T treated cells.

Tissue and cell extracts were prepared in RIPA buffer with Protease/Phosphotase Inhibitor Cocktail, Cell signaling Technology (#5872) according to the manufacturer's protocol.

For microarray analysis and cell culture assays cells were isolated with RosetteSep Monocyte Enrichment Cocktail (Cell Systems, St. Katharinen, Germany) according to the manufacturer's protocol.

Briefly, EE2 ECs or excised cremaster muscles were harvested using ice-cold IP lysis buffer containing complete protease and phosphatase inhibitor cocktail (Cell Signaling) and the samples were sonicated on ice three times for 5 seconds each.

For Western Blot of P1-tdTomato from erythrocytes, 20 μl of packed erythrocytes was lysed in ice-cold RIPA buffer (G-Biosciences, St . Louis MO) containing 1 100 Protease Inhibitor cocktail (Cell Signaling Technologies, Danvers, MA).

After centrifugation, cells were washed in 10 mL ice-cold PBS, and re-suspended in 100 μL of PBS supplemented with 1% protease inhibitor cocktail (Cell Signaling, #5871S), 1% phosphatase inhibitor 2 (Sigma Aldrich, #P5726), 1% phosphatase inhibitor 3 (Sigma Aldrich, #P0044), and 10% Cell Lysis Buffer (Cell Signaling, #9803).

For experiments in which we froze actin assembly and disassembly with the JLY cocktail, cells were allowed to spread on PLL coated glass for 30 min in the presence of 10 µM Rock kinase inhibitor (Y27632).

After washing twice with ice-cold PBS containing protease inhibitor cocktail, cells were collected in lysis buffer (1% SDS, 10 m M EDTA, and 50 m M Tris, pH 8.1), incubated for 30 min on ice, and then sonicated to shear DNA.

Briefly, cells were labeled with a cocktail of cell surface markers except the marker for the population of interest.

However, following the addition of a hormonal cocktail, the cells undergo further cell divisions.

inoculation with the peptide/TLR adjuvant cocktail, initial cell division time in response to peptide vaccine was uniformly ∼6 hrs for any combination of adjuvants and either route of inoculation (data not shown).