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On all coatings, cell area was the lowest on micropillars.
To achieve an array format compatible with majority of commonly used adherent cell types, different array surface materials were tested in combinations with different ECM protein coatings, cell dissociation procedures and adhesion incubation times for different cell types.
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For example, at the boundary of different extracellular matrix (ECM) coatings, cells tend to differentiate and selectively adhere to the area where the preferred ECM molecules reside [7].
The corresponding amount of TCN loaded in EPD coatings supported cell viability and proliferation of MG-63 cells for up to 3 days.
We present a simple approach for developing naturally derived ECM coatings for cell culture that provide important tissue-specific cues unlike traditional cell culture coatings, thereby enabling the maturation of committed C2C12 skeletal myoblast progenitors and human embryonic stem cells differentiated into cardiomyocytes.
REF52 cells were plated on cRGD-nanopatterns to assess the influence of the different cRGD coatings on cell adhesion behavior.
ECM protein coatings enhanced cell migration from liver explants cultured on PFPE or PDMS.
Also, the effects of ECM coatings on cell performance during extended time periods have not been addressed.
Results showed that iron oxide coatings increase cell deposition which was generally reversed by silver nanoparticle coatings in the early stages of injection.
The obtained flagellin variants were applied to create surface coatings inducing cell adhesion and spreading to different levels, while wild-type flagellin was shown to form a surface layer with strong anti-adhesive properties.
Biomimetic coatings with cell-adhesion-regulating functionalities are intensively researched today.
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