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For energy dispersive X-ray analysis (EDS, Quantax 200, Bruker, Germany), samples were coated with carbon in carbon coating unit and measurements were carried out at 20 kV at high vacuum.
Subsequently, they were mounted on stubs using colloidal coal as an adhesive, and sputter coated with gold (Polaron SEM Coating Unit E5000).
For the biofilm study, samples of the oropharyngeal section of the NGT were fixed overnight with 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4, washed with the same buffer, dehydrated in increasing concentrations of ethanol, dried with a critical point drier, and coated with gold (Polaron-sem coating unit E5100, Thermo VG Scientific, Beverly, MA).
The material was sputter-coated with gold in a SEM coating unit E5100 (Polaron equipment Ltd ,England) and stored over silica beads (Scharlau, Spain) in an air-tight container until imaged.
The cells were affixed to a metal SEM stub and sputter coated in gold by using an SEM coating unit (E5100 Polaron, UK).
Dried samples were mounted on stubs and sputter-coated with chromium in an EMS150T-ES coating unit (Electron Microscopy Sciences, Hatfield, PA, USA).
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A reel-to-reel continuous dip-coating unit was used to produce meter lengths of highly textured, crack-free Gd2O3 and LZO buffers on Ni and Ni–W tapes.
We applied reference-free, CTF corrected, subtomogram averaging to solve the structure of the repeating inner coat unit with a resolution of approximately 23 Å.
Indeed, a recent study reporting the cryo-electron microscope structure of the COPI coat (PDBid 5A1V) also suggests that the CTD of α-COP is one of the flexible connecting domains involved in oligomerization of coat units (triads) [ 37].
The mass thickness is the mass of the lead ditetradecanoate coating per unit area (and the thickness is this divided by the density).
A new photocatalytic reactor design for water treatment is presented, characterized by the use of new extremely narrow diameter lamps, thus allowing for much higher surface area for catalyst coating per unit reactor volume and consequently for much higher specific reactor capacity.
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