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Optimisation of the coating preparation parameters showed coatings obtained from [Rh(H2O 6](NO3 3 precursor dissolved in HNO3 1:2 (v/v) and fired at 430 °C for 2 h in a 5 l min−1 oxygen stream-furnished stable electrodes having the highest electrochemically active surface area.
We performed thorough characterization of the obtained surfaces after each step of the coating preparation and after mechanically challenging the obtained coatings.
On the other hand, the alloying effect in aluminide coatings depends on the element species, concentration, temperature, coating preparation technique, medium environment, and other factors.
Combinatorial coating preparation involves the synthesis of a large number of coatings using high throughput methods.
In terms of the quality of sprayed coatings, however, LPPS is still superior in terms of oxygen pick-up during coating preparation, which should affect the performance as a bond coating in service.
The lead coupon and coating preparation have been described elsewhere [13].
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A hybrid PVD device, which combined the HCD (Hollow Cathode Discharge) with the MS (Magnetron Sputtering) techniques, was utilized for the coating preparations.
Differences in URLs could result from the different coating preparations of the TPO antigen (purified native or recombinant) on solid phase, which affect the proper exposure of the immunodominant epitopes recognized by the polyclonal antibodies present in serum of patients with autoimmune thyroid disease (AITD).
In our opinion, these differences could lie in the different coating preparations of the TPO antigen (purified native or recombinant) on solid phase, which affect the proper exposure of the immunodominant epitopes recognized by the polyclonal antibodies present in serum of AITD patients (epitopic fingerprint), with the consequent lack of recognition of some of them [24, 25, 26].
Blood samples collected from participants at baseline and follow-up visits were subjected to density gradient centrifugation to yield a buffy coat preparation containing peripheral blood leukocytes and stored at −70C.
The blood sample collection was performed at the Division of Neurology, S.Francesco Hospital, Nuoro (I); the buffy coat preparation and the DNA extraction, according to classical salting out protocol, were performed at the Centro di Tipizzazione Tissutale of the Azienda Sanitaria Locale N°3, Nuoro, where the biological bank resides.
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