Sentence examples for coating plates from inspiring English sources

Stem cell induction began with hESCs UCSF-4 cultured in mTesR media (STEMCELL) on Cellstart (Invitrogen) coating plates.

Following protein coating, plates were blocked by adding 150 µl of 1% BSA in TBS for 1 hour at RT.

In order to obtain a suspension appropriate for coating plates, the milled form of the matrix was solubilized through enzymatic digestion [23], [24].

Antibody concentrations were determined by coating plates with goat anti-mouse IgG followed by incubation with mouse IgG1, IgG2b isotype controls (Sigma Aldrich) or IgG2c (Southern Biotechnology) isotype control followed by incubation with the corresponding HRP-conjugated secondary antibodies as described above.

After coating, plates were washed and incubated either with the rabbit antisera or with an anti-H5N9 chicken serum, generated by intramuscular vaccination of chicken with a commercial inactivated H5N9 poultry vaccine and kindly provided by our in-house vaccine registration department, diluted in washing buffer for 1 h at 37°C.

For both species, coating plates with MSP-1 resulted in higher sensitivity than PfGLURP or PvAMA.

A thick, boneless fillet of cod puttanesca was encased in a crisp, Parmesan-breadcrumb coating, plated with a generous mound of spinach and excellent roasted potatoes.

Immediately after cell preparation, CD4+ T cells were added to the coating plate (1 × 105 cells/well).

The AgNWs were aligned by the shear stress applied to the meniscus of a droplet of AgNW suspension injected between the deposition plate and the coating plate.

The protocol of preparing antigen and coating plate was according to the protocol described by Goris et al. [ 14].

VAKGL biotinated at either n- or c-terminus (0.5 µg/well) was coated onto streptavidin-coated plates Reacti-BindTM NeutrAvidinTM Highh binding capacity coated 96-well plate, Pierce).