Commercial coated-a-coat rat ELISA kits were provided by R&D Systems (Minneapolis, MN, USA).
PC12 cells were seeded at a density of 10 cells per well of a 4 well Lab-Tek II Chambered Coverglass (Thermo Fischer Scientific Nunc) coated with 0.1% of rat tail collagen I (Termo Fischer Scientific Gibco) in 30% ethanol.
A coating of type I rat-tail collagen allowed 87% (13/15) of embryos to attach to the matrix and 38% (5/13) to develop into egg cylinders (Supplementary Table S2).
Gels were coated with 0.5 mg/ml of rat tail type I collagen (Sigma) bound through the heterobifunctional crosslinker N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate (sulfo-SANPAH, Pierce).
PC-12 cells were grown on plates coated with 50 μg/ml of rat tail collagen I (Termo Fisher Scientific Gibco, Waltham, MA, USA) in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% horse serum, 5% FBS, L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml).
Lu, B. et al. Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system.
In addition, nano apatite coating significantly stimulated the proliferation and osteogenic differentiation of rat bone marrow stem cells.
The effects of micropatterned surfaces coated with type I collagen (CNI) on the proliferation and morphology of rat tail tenocytes were investigated in this study.
After isolation of rat neonatal cardiomyocytes, cells were grown on glass slides coated with a gelatin, as described previously [8].
After isolation of rat neonatal cardiomyocytes, cells were grown on glass slides coated with a gelatin as described previously [3], [15].
