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In co-culture experiments, NHEKs and NHEMs were seeded in 4-well culture slides pre-coated with Coating Matrix Kit (Life Technologies) at a density of 0.5×10 cells/well and 0.1×10 cells/well, respectively, in culture medium mixed with supplemented-PCT epidermal keratinocyte medium and supplemented-DermaLife® M Melanocyte Culture Medium at a 3 1 ratio as described elsewhere (Murase et al., 2013).
At 80% confluence, cells were trypsinized (Trypsin/EDTA, Toyobo) and subcultured onto culture plates coated with human Type-1 collagen (Coating Matrix Kit, Cascade Biologics).
The downgrading of electrical conductivity values of the composite coatings was due to the codeposition of insulating V2O5 and finer coating matrix.
The simplest approach for the preparation of anticorrosive nanocomposite coatings is through the direct mixing of inorganic nanoparticle additives with the organic coating matrix.
The hardness and wear resistance of the coatings were also improved due to the incorporation of Bi into the Ni coating matrix.
We therefore selected rLN511E8 as the coating matrix for our system.
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A finite element technique is used to study the effects of coating properties on the deflection and penetration of cracks which terminate perpendicular to the fiber-coating or coating-matrix interfaces.
The decrease of flexural strength during the tests is due to the debonding of the coating-matrix interfaces.
While if the fiber is harder and the coating is softer than the matrix, there is at least one unstable equilibrium position near the coating matrix interface, if the fiber is softer and the coating is harder than the matrix, there is at least one stable equilibrium position near the coating matrix interface.
The inclusion is taken to be perfectly bonded to the coating at the inclusion-coating interface while the coating matrix interface is assumed to be inhomogeneously imperfect of the spring-layer type.
In addition, effective moduli and CTEs of a composite fiber (fiber/carbon-coating) and those of a composite matrix (carbon-coating/matrix) were derived and used to interpret the resulted thermal stresses.
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