96 well plates were coated with coating antibody, as suggested by the manufacturer, incubated at 4°C overnight, blocked with 2% BSA as blocking buffer and incubated for 2 hrs at 37°C.
Black 96-well microtitre plates (Nunc Black MaxiSorp Surface; Nalgen Nunc International, Rochester, NY, USA) were coated with coating antibody at a concentration of 3 mg/ml of coating buffer (phosphate-buffered saline with 0.6 mmol/l EDTA) in a volume of 100 μl/well and kept at 4°C overnight.
Export of native proteins occurs at ER-specific sites where budding of vesicles coated with coat protein complex II takes place and by which they enter the Golgi complex (Fig. 3d).
Proteins are transported from the ER to the Golgi by vesicles coated with coat protein complex II (COPII), which was first identified in yeast and consists of the enzyme Sar1, and complexes made of Sec proteins (notably the heterodimeric Sec23/Sec24 complex, and the heterotetrameric Sec13/Sec31).
In co-culture experiments, NHEKs and NHEMs were seeded in 4-well culture slides pre-coated with Coating Matrix Kit (Life Technologies) at a density of 0.5×10 cells/well and 0.1×10 cells/well, respectively, in culture medium mixed with supplemented-PCT epidermal keratinocyte medium and supplemented-DermaLife® M Melanocyte Culture Medium at a 3 1 ratio as described elsewhere (Murase et al., 2013).
l-Carnitine-loaded compact pellets were prepared by extrusion method and then coated with various coating materials such as ethylcellulose (EC), Eudragit E100 (EudragitudRS100 RstearylS100), stealcoholcohol and glyceryl monostearate, for single-layered coated pellets (SCP).
An experimental investigation was conducted to evaluate the compressive strength, modulus of elasticity and carbonation resistance after heating of air-cooled concrete coated with surface coating of concrete (SCC).
Titanium alloy rods (Ti6Al4V, 2.5-mm diameter and 45-mm length) were plasma spray coated with HA (coating thickness = 30 μm, Ca/P = 1.67, Biomaterial Centre of Sichuan University, Sichuan, China).
A CDMPC-coated CSP with coating quantity of 15% was thus prepared.
Following synthesis in the endoplasmic reticulum, trans-membrane and soluble proteins co-translationally inserted into the ER are packaged into transport vesicles coated with COPII (coat protein complex II) for export from the ER and delivery to the Golgi for further processing (Lee et al., 2004).
Coated kernels presented reduced moisture absorption during 120 days of storage (especially those coated with the nanocomposite coating), indicating that the coatings could decrease the texture changes of kernels.
