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Methocel® E50 was found to afford the best balance among different important items, i.e. process time, retarding ability, dimensions of the coated units and possibility of finely tuning the delay duration.
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Subsequently, they were mounted on stubs using colloidal coal as an adhesive, and sputter coated with gold (Polaron SEM Coating Unit E5000).
The cells were affixed to a metal SEM stub and sputter coated in gold by using an SEM coating unit (E5100 Polaron, UK).
Dried samples were mounted on stubs and sputter-coated with chromium in an EMS150T-ES coating unit (Electron Microscopy Sciences, Hatfield, PA, USA).
The International Paper Company said yesterday that it had agreed to sell its coated paper unit to a private equity firm, Apollo Management, for $1.4 billion.
c Top view of cell-coated hydrogel units assembled in a fluidic channel.
Finally, we demonstrated in vivo-like 3D environment by assembling cell-coated hydrogel units in the fluidic channel.
In addition, in vivo-like cell culture environment was demonstrated using an assembly of cell-coated hydrogel units in the fluidic channel.
In addition, an assembly process of cell-coated hydrogel units can be provided simple construction of a 3D cell culture environment under fluidic conditions.
To verify a 3D cell culture environment on a chip under flow conditions, cell-coated hydrogel units were assembled in a fluidic channel.
a, b Side view (a) and top view (b) of a cell-coated hydrogel unit.
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