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The coated support was heated at 80°C overnight.
The coated support was then heated at 80°C overnight for FA polymerization.
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A dynamic mechanical thermal analysis (DMTA) performed on stacked coated supports or on samples sandwiched between rigid supports (carbon or glass fibers, metal or glass plates, …) is described.
This work describes an alternative method to selectively immobilize probes and to detect DNA on biocompatible, hydrophilic cellulose coated supports with low unspecific binding, high selectivity and appropriate sensitivity.
Methanol solution of ZIF-8 and enzyme/ZIF-8 composite was placed on the carbon-coated support grid and dried at room temperature for TEM measurement and energy dispersive X-ray spectrometry (EDS) analysis on a JEOL JEM-2010 high-resolution TEM with an accelerating voltage of 120 kV.
Primary muscle fibroblasts from two different healthy individuals and two FSHD patients with 5.5 D4Z4 repeats and 7 repeats [14], [29] in the 4q35 array, respectively, were cultured on a collagen-coated support in DMEM supplemented with 20% bovine fetal serum.
Formvar-carbon-coated support films were then floated onto distilled water, after which grids were placed onto the film before lifting onto parafilm and air-drying.
After the separation of the two vault halves, the external acidic surfaces observed in the R1 R7 region (Supplementary Figure 1C), would try to maximize their electrostatic interactions with the polylysine-coated support.
Samples were mixed 1 1 with 10 nm protein A-gold (Aurion, Wageningen, The Netherlands) as fiducial markers and applied to glow-discharged R2/2 Cu 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) by gentle pipetting.
The zeolite-coated supports were characterised by means of XRD and N2 adsorption measurements.
The influence of the binder on the properties of the zeolite-coated supports such as surface area, micropore volume and adhesive strength was investigated.
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