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These cells exhibit an accumulation of clathrin-coated vesicles and an increase in U-shaped clathrin-coated pits, which may result from sequestration of coat components on uncoated vesicles.
Such phenotypes were reminiscent of the accumulation of puncta positive for endocytic clathrin coat components observed in cells where the progression of late stage clathrin-coated pits to free vesicles is impaired (Ferguson et al., 2009; Posor et al., 2013), strongly suggesting that lack of OCRL affects the completion of the endocytic reaction.
Endocytosis inhibition is caused by a dramatic increase in the lifetimes of clathrin coat components, including FCHo, clathrin, and dynamin, suggesting that the clathrin TD regulates coated pit dynamics.
Cargo adaptors typically link membrane proteins to coat components.
Egg coat components polymerize using a common zona pellucida (ZP) "domain" module that consists of two related immunoglobulin-like domains, called ZP-N and ZP-C.
In addition, the tendency of following zero order kinetics was mainly affected by the coat components rather than those of the core.
Similar(15)
Sec16 is an early determinant of tER site biogenesis and exhibits a localization that is undistinguishable from that of COPII coat component Sec23 [21]; therefore, it was used as a tER site marker in all our subsequent experiments.
Yeast AP-2 complex is also an early coat component.
We report the identification of a novel postovulatory coat component in marsupials, which we call uterinesecreted microprotein (USM).
The red channel shows immunostaining for ERGIC-53; YKT6 (after cytosol washout); early and late Golgi markers (Mannosidase II and TGN46); and the COPII coat component Sec31.
Binding and colocalization with Sec31A, a coat component of the ER to Golgi transport vesicle COPII were found to be Ca2+-dependent [14 16].
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