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Bleeding was very common and associated with mortality in leukaemia patients in the ICU, but it was difficult to predict in the individual patient using traditional coagulation analyses.
All potential biological coagulation analyses, however, require that the patient has been admitted to the hospital, and they are time-consuming.
Considering standard laboratory coagulation analyses, the highest predictive values for MT were provided by hemoglobin, Quick value and aPTT (ROC-AUC values shown in Table 4).
For the coagulation analyses, blood was initially collected in 3.5 mL tubes containing 0.35 mL buffered 3.2%% trisodium citrate (Vacuette; Greiner Bio-One, Linz, Austria), giving a volume ratio for citrate to whole blood of 1 9.
As the coagulation analyses and the haemostatic therapy were part of the clinic's standard, the ethics committee waived the necessity to obtained informed written consent from the patients included in the analyses.
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Blood coagulation status was assessed by PT INR, analysed with CA-7000 automated coagulation analyser (Sysmex Co., Kobe, Japan).
Citrate blood samples for coagulation profile analyses were collected together with the routine laboratory testing for blood count, C-reactive protein (detection limit 0.2 mg/l) and interleukin 6 within the first hour of life prior to vitamin K administration (0.2 mg phytomenadione solution intravenously).
The expression levels of genes involved in PA biosynthesis and tannin coagulation were analysed, and some of them were verified using quantitative real time PCR (qRT-PCR).
> Pre-treatment, there were no between-group differences in the activities of the other recorded coagulation factors analysed (FII, antithrombin, FV, FVIII and FXIII; Fig. 2 a– e; Table 2).
Complete blood count, blood biochemistry tests, coagulation test, urinary analyses and anti-ADI-PEG 20 antibody titre were assessed after every cycle (4 weeks) of treatment.
Patients were evaluated prior to enrollment by means of: medical history; physical examination; assessment of ECOG PS; complete blood count with differential; complete metabolic panel analyses; coagulation parameters; BM aspirate assessments; and a biopsy, including flow cytometry and cytogenetic analysis.
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