This striking feature raises the question as to how T. gondii generates the mitochondrial acetyl-CoA to be fed into the TCA cycle.
Due to the prohibitive cost of cerulenin, the simultaneous overexpression of both the acetyl-CoA carboxylase (ACC) gene and the biotin ligase (BirA) gene from Photorhabdus luminescens was obtained allowing acetyl-CoA to be more efficiently converted to malonyl-CoA.
The CoA thioester of the C7H7NO3 acid presumably corresponds to hydroxyanthraniloyl-CoA. Interestingly, 5-hydroxyanthraniloyl-CoA was reported to be formed in the reaction catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase under NADH-limiting conditions [ 77].
The currency metabolites HEXT, PI, ADP, ATP, NAD, NADH, Q, QH2, NADP, NADPH, FAD, FADH, UTP, and COA are considered to be unconditionally available in the cell.
The carbon flux from pyruvate to acetyl-CoA was determined to be 24.7, which was 12.27% higher than that in the batch culture.
The feruloyl-CoA was suggested to be a major precursor in scopoletin biosynthesis [ 15].
In vitro, feruloyl-CoA was reported to be converted to the corresponding stilbene, albeit at low levels[ 31].
For BlmB, the KM for acetyl-CoA was determined to be 19 μM, which corresponded well with the previously reported value of 12 μM.
The condensation reaction of vanillylamine with 8-methyl-6-nonenoyl-CoA is thought to be catalyzed by a coenzyme A-dependent acyltransferase.
(S -3-Hydroxyacyl-CoA dehydrogenaS -3-Hydroxyacyl-CoAured in both directionS -3-Hydroxyacyl-CoAoA and 3-hydroxybutyryl-CoA. FadB' waS -3-Hydroxyacyl-CoAly stereospecific to (S)-3-hydehydrogenaseCoactivity prefer NAD+.
