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The cox genes enable CO utilization under aerobic chemolithoautotrophic conditions by catalyzing the oxidation of CO to CO2[1], [15], [16].
Based on these findings, we decided to test directly for CO utilization.
Members of the Roseobacter lineage are involved in aerobic anoxygenic photosynthesis, dimethylsulfoniopropionate (DMSP) degradation, and CO utilization in marine surface waters [2].
We delineated the genetic determinants of the P7T metabolic pathways for CO utilization and characterized a complete Wood-Ljungdahl pathway gene cluster in its chromosome.
To identify the sets of genes responsible for CO utilization and for acetate, butyrate, ethanol and butanol production, the P7T genome was sequenced and partially annotated.
The genetic elements involved in this microorganism's metabolic pathways for CO utilization, acetate and butyrate as well as ethanol and butanol production, were compared with those from other solvent-producing Clostridia.
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It was later observed that this set-up also favored co-utilization of xylose when recombinant xylose-utilizing strains of S. cerevisiae were used (Olofsson et al. 2008b; Öhgren et al. 2006).
Reider Apel, A., Ouellet, M., Szmidt-Middleton, H., Keasling, J. D. & Mukhopadhyay, A. Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae.
As a consequence, both approaches result in the strains for effective co-utilization of glucose and glycerol.
With help of this model, an SSCF process with efficient co-utilization of glucose and xylose was successfully designed.
Co-utilization of fossil fuels and biomass is a successful way to make efficient use of biomass for power production.
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