We extracted RNA with the TIANamp RNA Extraction Kit for Virus Detection (cat. NO DP315-R; Tiangen Biotech Beijing Co., Ltd, Beijing, China), or the TIANamp N96 Virus RNA Kit (cat. NO DP434; Tiangen Biotech Beijing Co., Ltd) in semihigh-throughput operations.
In this section, we take an empirical application on a series of daily stock price of LBC (SHANDONG LUBEI CHEMICAL Co., LTD) in the Shanghai Stocks Exchange.
After 2, 6, 12, and 24 h of incubation, the adhered cells on experimental samples were washed with 1 × PBS and fixed with 2.5%% w/v glutaraldehyde (Sinopharm Chemical Reagent Co., Ltd) in 1 × PBS for 4 h.
The relationship between the soil water content and pF value of each soil was measured using the wide range pF meter (Model: DIK-3404) produced by Daiki Rika Kogyo Co., Ltd in Japan.
The algal powder was mixed with 3 mL of pre-cooled extraction buffer (50 mM phosphate buffer, pH 7.2, 0.1 mM EDTA) and homogenized using an S10 homogenizer (Ningbo Xinzhi Biotechnology Co., Ltd) in an ice-water bath (Nagarani and Kumaraguru 2012).
Electricity is distributed by Brihanmumbai Electric Supply and Transport (BEST) in the island city, and by Reliance Energy, Tata Power, and Mahavitaran (Maharashtra State Electricity Distribution Co. Ltd) in the suburbs.
The Duroc, Landrace, and Yorkshire pig samples were collected at the NIAS and Sunjin Co., Ltd in Korea.
Fruit firmness was determined on four directions of each fruit using a DESIK-GY-4 penetrometer (Zhejiang Top Instrument Co., Ltd) in 2010 and 2011.
Systolic blood pressure (SBP) was measured at the end of treatment using non-invasive tail-cuff apparatus (Bionics Instrument CO., LTD) in anaesthetized mice.
The protocol of Argon laser photocoagulation GYC-10000, Nidek, Co., Ltd) in our clinic was as follows: at first, the laser targeted for bare sclera detached from ciliary body.
Tumour-bearing rats were administered either Nω-nitro L-arginine methyl ester (L-NAME, Sigma-Aldrich Fine Chemicals) or S-methylisothiourea sulphate (SMT, Wako Pure Chemical Co., Ltd) in 0.2 ml of 0.9% NaCl solution, given intraperitoneally (i.p). at a dose of 6 mg kg−1 per day for 5 days, beginning 9 days after tumour implantation.
