If AMPARs and NG2 are associated and co-inserted at the synapse, LTP in NG2 cells would increase local expression of NG2 at single neuron-to-NG2 cell synapses.
To develop a more efficient procedure to identify a complementary cell line for screening, we utilized an alternative reporter design in which a secreted nanoluciferase reporter (secNLuc) was co-inserted with a GFP reporter to allow cell sorting of GFP-expressing clones.
It was observed that aluminium had a tendency to wet uncoated carbide (94%WC + 6%Co) inserts.
After administration of sedatives and local anesthesia, a 17-gauge cooled-tip electrode (Cool-Tip; RF Ablation System, Covidien, Boulder, Colombia, CO) was inserted.
One to two fully expanded leaves were removed from each shoot and placed in a mesh insert (Life Science Products, Inc., Frederick, CO, USA) inserted in a microcentrifuge tube and immersed in liquid nitrogen until completely frozen.
To generate GFP-FCHO2, full length human FCHO2 (1 810) was PCR amplified from cDNA clone #9021067 (Open Biosystems, Lafayette, CO) and inserted into pEGFP-C1 using SalI restriction sites and Cold Fusion cloning technology (System Biosciences, Mountain View, CA).
A 2F micromanometer-tipped transducer (Millar Instruments, Houston, TX) connected to a PowerLab (8/30) system (ADInstruments, Colorado Springs, CO) was inserted into the right carotid artery to record systolic and diastolic blood pressure.
After manometry, a calibrated standard antimony-based pH catheter (Comfortec Plus catheter and ZepHr Sleuth recorder, Sandhill Scientific, Inc., Highlands Ranch, CO) was inserted trans-nasally to 5 cm above the proximal border of the LES, affixed to the nose, and connected to the data recorder.
Through a co-self-assembling peptide of phosphorylated serine peptide amphiphile and RGDS peptide amphiphile, Stupp and co-workers inserted the peptides based nanofiber into a 5 mm rat femoral critical-size defect to demonstrate bone formation and mineralization within 4 weeks [59].
Wang and co-workers inserted an insulating tunneling layer between the perovskite and the electron transport layer.
