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A Marianatm system (Intelligent Imaging Innovations, Denver, CO) consisting of an Axiovert 200M epifluorescence microscope (Carl Zeiss, Thornwood, NY) with a spinning disk confocal head (CUX-22, Yokagawa, Japan) was used for spinning disk confocal imaging.
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Exposure of cells to nanoparticles was performed using stock suspensions of 300 μg/ml WC (in water) and 330 μg/ml WC-Co (consisting of 300 μg/ml WC and 30 μg/ml cobalt, in 0.03% sodium polyphosphate, see Bastian et al. [ 21]).
We expected the similarity in BET because WC-Co consists of 90% weight WC powder and both were milled under equal conditions.
Then, we analyze a process model of value co-creation consisting of four phases, i.e., co-experience, co-definition, co-elevation and co-development.
SMARTpool siGENOME (Dharmacon by Thermo Fisher Scientific, Lafayette, CO, USA), consisting of four siRNA duplexes, were used to target SRSF4 and SRSF6 mRNA.
Interestingly, WC-Co particles (consisting of 30 μg/ml WC and 3 μg/ml cobalt) showed a higher toxicity than equivalent concentrations of CoCl2 (3 μg/ml) indicating that leaching of cobalt alone may not explain the toxic effects.
The apartment is one of 55 units in a co-op consisting of three turn-of-the-century town houses.
The system allowed formation of P 3HB-co-4HB) consisting of 1–9 mol% 4HB.
H. bluephagenesis was designed to produce a microbial copolymer P 3HB-co-3HV) consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV).
The utility of our approach was demonstrated through the fabrication of a patterned co-culture consisting of micropatterned neuroblastoma cells surrounded by immobilized myeloid cells.
A random copolymer, poly NIPAAm-co-PtPorphyrin), consisting of N-isopoly NIPAAm-co-PtPorphyrinand poly NIPAAm-co-PtPorphyrinits, behaves as an opticonsistingensof for oxygen and temperature.
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