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Recent findings have shown that cardiac output (l/min) is lower in CMS when compared with sea-level (SL) dwellers.
Molecular marker studies showed a statistical increase in DNA mass and calponin expression after 3 and 7 days of CMS when compared to static samples, indicating that the translation of mechanical loading from the novel polyurethane elastomeric scaffold onto VSMCs will be important to consider with regard to modulating cell phenotype.
However, all parameters tested were unaffected by topically applied CMS when compared to vehicle-treated controls.
LSF provided better postoperative shoulder function (CMS) when compared to HP fixation, but the difference was not statistically significant.
Cav1.2, which is a major subunit of Ca2+ channel expressed in CMs, showed an increased level in HCM CMs when compared with control CMs.
Another aspect of our work is noteworthy: we measured very modest APD prolongation in both LQT2-hiPSCN996I and NKX2.5 eGFP/w hESCN996I CMs when compared to their wild-type counterparts, with values shorter than those reported in other LQT2 hiPSC models (Itzhaki et al, 2011; Matsa et al, 2011; Lahti et al, 2012), and did not observe any early-after depolarization in the mutated cells.
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Consistent with this observation, we found an increased level of nuclear NFATC4 in HCM iPSC-CMs when compared with control iPSC-CMs via immunostaining.
The major goal is to generate iPSC-cardiomyocytes (iPSC-CMs) from LVAD patients and evaluate the genotypic and phenotypic fidelity of iPSC-CMs when compared to native CMs.
It was found that the electrical resistivity decreased from 6.68 mΩ-cm to 1.63 mΩ-cm when compared with undoped Bi2Te3, at room temperature.
The left ventricular wall thickness was significantly greater, however, in the group with elevated PAOP (1.37 ± 0.04 cm) when compared with the group with normal ventricular filling pressures (1.05 ± 0.15 cm) (p = 0.001).
In vitro scratch assays were used to evaluate the potential of MDC and AFDC CM to promote epithelial cell migration, and we found that MDBK cells migrated faster in the presence of MDC, but not AFDC, CM when compared to epithelial cells in DMEM (Fig. 5bi & ii).
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