A PCR fragment amplified with JVO-10927/JVO-10668 from pWRG100 was first integrated into the gltI- sfgfp KmR strain, and next, the CmR I-SceI allele was replaced by an 80-mer dsDNA of JVO-10966/JVO-10967 using pWRG99.
Briefly, the E. coli strain to be modified was initially transformed with plasmid pACBSR (CmR), expressing I-SceI and λ Red proteins under the control of PBAD promoter (inducible with l-arabinose), and subsequently electroporated with the corresponding pGE-based suicide vector (KmR).
The more relevant need had been to search for ways of testing the validity of the probabilistic CMR hypothesis, i.e., the one that the authors had actually formulatedh.
Given the nature of the intervention, it is not possible to blind patients and investigators to the study intervention; however, the radiologists assessing the cMR studies (i.e. outcome assessors) and data analysts will be blinded to patient allocation.
DNA fragments containing a CmR resistance marker and a I-SceI recognition site amplified with primer pairs JVO-0303/JVO-0304 and JVO-7448/JVO-11078 from the template plasmid pWRG100 were integrated into the chromosomal sroC and gltIp region, respectively, by lambda Red recombinase expressed from pKD46 (Datsenko & Wanner, 2000).
Second, patients investigated with scanner I had CMR during breathhold, while patients in the second cohort had CMR during free breathing.
A significant response was classified as a reduction of at least 50% in TLG between baseline and follow-up (i.e. CMR + PMR).
The greatest advantage of cross-sectional imaging (i.e., CMR and CCT) over echocardiography is the information available on extracardiac structures.
First, a kappa test was performed to measure the intra-observer and inter-observer concordance for metabolic response (i.e. CMR, PMR, SMD and PMD) between the EORTC and PERCIST criteria.
Delayed CMR was defined as CMR after second-line chemotherapy.
