The study was approved by the local ethics committee (Commissie Mensgebonden Onderzoek – Regio Arnhem Nijmegen, CMO 2001/095), and a written informed consent was obtained from the subjects according to the Declaration of Helsinki.
The study was approved by the Committee on Medical Research Involving Human Subjects CMOO) Arnhem-Nijmegen (Filenr. CMO 2007/172).
The Medical Ethics Committee of the Radboud University Medical Center formally stated that this study was exempt from ethical review (Radboud University Medical Center Ethical Committee review reference number: CMO: 2010/244).
NfsB-catalyzed reduction of the 4-NB group in ntla cMO 10a should generate a 4-hydroxylaminophenyl intermediate, which will rapidly undergo 1,6-elimination to cleave the carbamate linkage.
This study has been carried out in the Netherlands in accordance with the applicable rules concerning the review of research ethics committees and informed consent (The Research Ethics Committee of the Radboud University Nijmegen Medical Centre, Filenumber CMO 2012/412).
The 5′ amine- and 3′ disulfide- modified oligonucleotide (8a, 5′-GACTTGAGGCAGA CATATTTCCGAT-3′; start codon underlined) was coupled with the linker in aqueous buffer to obtain the linear intermediate 9a, and disulfide reduction with immobilized triscarboxylethylphosphine (TCEP) yielded the 4-NB ntla cMO 10a through intramolecular cyclization.
hdMO1, hdMO2, cMO and mcMO1 morpholinos were prepared and injected in the manner described in ref. (18).
Also, the voltages on V in +, V in −, and V cmo are 1.7, 1.3, and 1.5 V, respectively, to have 1.45 V of a common-mode voltage at the sample and hold stage.
Some important drought-related genes have been cloned using RT-PCR and the RACE method, including the CMO [ 13], ARF1 [ 14] and EF-hand CaBP [ 15] genes.
To enable such automation, the content element could direct to a very semantically defined and structured file type eg CML(and its extensions) [48], CIF [49], AniML [50] or to an ontology eg RXNO, CMO, MOP [51], ChEBI [52] or a structured vocabulary such as CSMD [33].
