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GdnHCl and urea induced unfolding of BmTMK is non-cooperative and influence the functional property of the enzyme much lower than their Cm values.
Table 1 indicates that our measured ρ 300 K) ≈ 310 to 350 μΩ cm values are a few times higher than that (≈ 60 μΩ cm) reported for bulk TiSi [3].
The averaged CM values range from 0.45 to 1, indicating highly correlated motions.
The generally lower CM values in Figure 5b indicate weaker dimeric interactions.
However, non-zero CM values were also obtained when no apparent causal effects were evident, or no direct connections were present among the considered neurons.
The PPC in Figure 3C was built by comparing the sorted SWM and CM values, from the highest to the lowest (white squares, cf., Figure 3D).
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The Cm value for F4A and Y343A was calculated to be 4.25 and 3.0±0.05 M, respectively.
Hence, we retrieved cM value by means of linear interpolation between the two closest markers with known genetic distance.
The Cm value for ΔQ2, Q2R, and ΔP3 mutants was calculated to be 21.85, 19.84 and 17.94±0.05 M, respectively.
The Cm value for F4W was determined to be 5.0±0.05 M. At concentrations of urea below 2 M, F4A and Y343A seemed resistant to proteolysis.
No cM value was attached to the locus but its coordinates on Os5 were given (Additional file 2, Table S1).
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