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Quartz cells (1.0 cm path length) from Hellma were used.
The therapeutic window was adjusted by using 10% KI (5 cm path length) and pyridine (1 cm path length) as a filter for UV radiation.
UV-vis absorption spectra of samples were measured on a (spectro 2060 plus spectrophotometer over 200 800 nm using 1 cm path length cuvette-UV analysis) equipped with a 1-cm path length quartz cuvette.
The reduction took place in aqueous solution in a standard quartz cell with 1 cm path length.
The samples were placed in silica cuvettes (1 cm path length), using acetone as a reference solvent.
The fluorescence spectra were measured at 25±0.1°C with a 1 cm path length cell.
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Absorbance measurements were taken in 1 cm path-length cuvettes.
The fluorescence spectra measured were on a Hitachi F-2500 spectroflusingeter using a 1 cm path-length curvette.
Far-UV circular dichroism (CD) was recorded on a Chirascan spectrophotometer using a 0.1 cm path-length cell.
Measurements were obtained in Genesys 10UV spectrophotometer (Thermo Scientific, Waltham, MA) with a 1 cm path-length cell.
The samples were then centrifuged at 42000 rpm using two-sector 1.2 cm path-length carbon-filled Epon centerpieces.
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