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Here, a wire grid (8 × 14 cm) is fixed on an electric balance.
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The biopsy (0.3 × 0.2 × 0.1 cm) was fixed in 4% formaldehyde and processed entirely in paraffin, and multiple sections were cut and stained with hematoxylin and eosin as well as Brown and Brenn tissue gram stain, Ziehl-Neelsen stain, and Fite's acid-fast stain for mycobacteria, and Grocott's modified Gomori's methenamine silver stain for fungal elements.
A grey plastic square (9 cm × 9 cm) was fixed to the base of each cylinder.
A gray plastic square (9 cm x 9 cm) was fixed to the base of each cylinder.
Small portions (0.5 cm × 0.5 cm) were fixed immediately in 10% buffered par formaldehyde (pH 7.2) and embedded in paraffin.
The other segment (1 cm) was fixed in 10% buffered formalin and processed for paraffin block preparation.
A podium (10 × 4 cm) with a recessed food plate (diameter 3 cm) was fixed at 1.5 cm from the end of each of the maze arms.
Dorsal skins (1.5 cm × 1.5 cm) were fixed in 10% formalin neutral buffered solution, embedded in polyester wax and sectioned at 6 mm.
Tissue isolation was carried out as follows: the proximal 7 cm was fixed overnight in methacarn (methanol, chloroform and acetic acid; 4 : 2 : 1) and then paraffin embedded.
Tissues obtained 0.5 1 cm were fixed with 4% formaldehyde, embedded in paraffin using standard procedures, and routinely cut into sections (5 um-thick).
Excised liver specimens from different lobes (1 cm × 1 cm) were fixed in 10% paraformaldehyde, embedded in paraffin, sectioned and stained with hematoxylin and eosin (H&E), Masson's trichrome or silver.
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