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The point is then quantified by measuring the distance in millimetres between "No Pain" and the point marked by the woman against a 0-10 cm gauge.
Bone marrow aspiration was performed, using a 13,5 cm gauge bone marrow needle (Jamshidi bone marrow aspiration needle), under sedation with midazolam and ketamine; 1, 2 ml of bone marrow were aspirated into the syringe and then smeared on 8 slides (4 fixed with alcohol and 4 without fixation) that were air dried and sent to the pathology laboratory for cytological analysis.
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ST36 was needled bilaterally perpendicularly to a depth of 2 mm with a 1.27 cm 32 gauge acupuncture needle (U Kwong Co Ltd, Taipei, Taiwan).
Non-directed bronchoalveolar lavage was performed by instilling 20 ml of sterile 0.9% saline via a standard 50 cm, 14 gauge tracheal suction catheter as described previously [ 16, 17].
Blood samples (1 mL) were taken from the caudal vein with a 1.9 cm, 26-gauge needle and a syringe previously rinsed with a solution of heparinized saline to prevent coagulation (Houston 1990; Russell 1990).
In order to obtain adipose tissue venous blood, a 10 cm 22-gauge catheter (Central venous catheter kit Seldinger technique; Becton Dickinson, Alphen aan den Rijn, the Netherlands) was introduced anterogradely over a guide wire into one of the superficial veins and threaded towards the groin, so that its tip lay just superior to the inguinal ligament [ 15].
In each insertion procedure, CT was used to guide a 15-cm, 18-gauge double-wall Chiba needle (18 patients; Cook, Bloomington, IL) or a 15-cm 19-gauge Temno guiding double-wall needle (10 patients; Cardinal Health, Dublin, OH) into the lung lesion (Fig. 1).
A 7/8-inch (2.2-cm) 25-gauge needle is inserted until bone contact.
A 1.5-inch (3.8-cm) 25-gauge needle connected to a syringe of lidocaine is inserted.
A 1.5-inch (3.8-cm) 22-gauge needle is inserted until bone contact and the injection is tested with lidocaine.
A 1.5-inch (3.8-cm) 25-gauge needle is inserted in a slight caudocranial direction upon bone contact.
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