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Tomographic visualization and electron microscopy of immunogold-labeled SEA-treated B cells revealed small clusters of surface HLA-DR1 (≤4 gold labels).
Examination of surface conservation among 10077a d revealed well-defined clusters of surface residues (Figure 5), including Val10, Thr13, Glu14, Asp15, Lys18, Val19, Ala22, Asn25, Ile63, Asp65, Ala67, Arg68, Lys86, Gln87, Ala89, Asn95, Ile104, Pro125, Thr127, Gly130 (using 10077a residue numbering).
HEK293 cells were transiently transfected with IGF1R-fl (WT), IGF1R bearing the kinase-inactivating mutation D1105A (KD), IGF1R with clusters of surface mutations (C1-C6), or not transfected (U).
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Incubation at room temperature prevents intracellular translocation of antibody-nucleolin complex and allows antibody-dependent clustering of surface nucleolin [6].
The efficiency of energy transfer is especially high at the higher dose of SEA, indicating substantial clustering of surface DR1 at this dose of SAg.
The addition of the control SAg SEH does not increase clustering of surface DR1 on B cells in the presence or absence of EDTA (Fig. 2C, ii, iii).
The cross-linking of cell surface bound HB-19/Btn with anti-biotin antibodies results in ligand-dependent clustering of surface nucleolin and its colocalization with the pseudopeptide, thus confirming that surface nucleolin is the target of HB-19 [12].
We propose the following model: tetherin-mediated virion retention triggers clustering of surface tetherin, targeting it for endocytic uptake.
CD95-dependent apoptosis is triggered by CD95L (FasL, APO-1L, TNFSF6) binding and clustering of surface CD95.
For Pfeiffer's Cave (near the type locality of E. latitans), both groups are well separated from each other, but also from the main cluster of surface forms.
The surface nucleolin in the 500-kDa complex should be associated, directly or indirectly, to an integral membrane protein partner that holds this complex to coordinate clustering of surface nucleolin along with active endocytosis of various nucleolin-binding ligands via lipid rafts [ 10, 17, 19].
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