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Individual gold labels cannot be distinguished at this magnification, but clusters of labels are visible as bright spots in the image.
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One more element is required to fully reproduce every aspect of the clustered labeling patterns seen in cortex: a rule is needed that produces discrete clusters of label far from a large injection site.
In contrast, following 2-AAF treatment the proportion of labeled cells dropped and only rare large clusters of labeled hepatocytes were detected.
The auditory shelf directly ventral to the medial ∼2/3rds of HVC that shows auditory-induced expression (Fig. 6A, 3rd and 4th row) did not show any clusters of labeled neurons in the hopping deaf animals (Fig. 6C; Fig. S4Aa).
Small injections would result in clusters of labeled somata but diffuse anterograde labeling of boutons.
Clusters of labeled cells were considered clonal if they were clearly distinct and at least 100 µm away from other labeled cells.
Small injections into all other regions across the cortical surface produced colocated clusters of labeled boutons and somata (Fig. 6 c,d —arrowheads indicate corresponding clusters).
Models II and III produced clusters of label in predefined locations across the cortical sheet, irrespective of the location of each injection.
The fact that large injections result in discrete clusters of label at all is surprising, when one considers the functional properties of primary visual cortex.
One report of injections in posterior IT cortex has described multiple clusters of labeled cells within V4 (Felleman et al. 1997).
Four distinct, scattered, clusters of labeled cells are present throughout trunk and neck, including a single yellow clone in ventricle (1, arrowhead).
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