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The resulting clusters of alignments were then processed by PASA [ 52].
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Clusters of alignment assemblies were mapped to existing genome annotations as previously described, using a 1% overlap criteria instead of the original 40% employed.
All clusters of PASA assemblies correspond to single-linkage clusters of alignment assemblies having identical transcribed orientations and overlapping by at least 50% of either alignment's genome span.
Sorting discordant alignments by chromosome coordinate allows the discovery algorithm to 'sweep' across chromosomes in search of clusters of discordant alignments that support a common SV breakpoint.
Clusters of duplicate alignments were then extracted by grouping all reads with the same prefix and aligned position.
Our analysis was hereby confined to those clusters of PASA alignment assemblies including multiple assemblies considered to be full-length (assemblies including at least one FL-cDNA).
At each genome site occupied (though not necessarily bound directly) by a protein, ChIP-seq produces a tight cluster of read alignments, which can then be detected by software with high resolution.
Socrates works by efficiently identifying clusters of soft clipped alignments in the reference genome as candidate break points.
Each cluster of PASA alignment assemblies containing more than one alignment assembly was a direct result of at least one splicing variation.
The phylogenetic analysis for the MLST tree is based on the identified informative sites among the seven housekeeping genes, for the pan-genome tree on presence and absence of genes and for the consensus tree based on the informative sites of core gene clusters from alignments of all core genes trees.
Each cluster of PASA alignment assemblies can contain either single or multiple transcript alignment assemblies.
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