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Unigene sequences from the crop expressed sequence tag database CR-EST (clustering project g03) served as template for primer design using Primer3 [ 33].
For the sequences identified in the CR-EST database (clustering project g03) http://pgrc.ipk-gatersleben.de/cr-est/, GeneRunner software http://www.generunner.net was applied to design PCR primer pairs each amplifying a 300-600 bp fragment from an individual EST.
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