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The tight clustering of tissues enabled the identification of CpG loci with tissue-specific DNA methylation profiles.
To our surprise, the clustering of tissues using the miRNA expression profiles was very similar to that obtained by the mRNA expression profiles.
Based on the clustering of tissues in Figure 4, it appears as if this extends also to the transcription of HERV-W elements.
Clustering of tissues by only their overall gene expression patterns suggested that floral tissues, the bud samples, and root and xylem had the most similar gene expression profiles (Additional file 13).
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However, unsupervised classification or clustering of tissue samples should also be studied since in many cases, labeled tissue samples are not available.
Similarly, the tight clustering of tissue types having related biological functions is striking.
Complete linkage clustering of tissue-specific miRNA expression using the Pearson correlation distance was performed using Genepattern [ 58].
The entire dataset shows clustering of tissue samples regardless of the developmental stage suggesting early completion of the transcriptional programs in the minipig (Fig. 4a).
Clustering of tissue-specific genes suggests that there exists some higher order in regulation of gene expression, most probably due to the chromatin domain.
Conceptually, sequence could be the major driving force influencing the epigenetic framework, which, if true, could help explain the persistence of genome-scale epigenetic modifications and the observed clustering of tissue-specific or generalized functions in complex genomes.
Logarithmic transformation again serves to improve the clustering of tissue types to some extent, but there is still systematic variation in the raw data that remains problematic even after removal of potential bias from differences between bioinformatics pipelines.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com