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The p-value is then the fraction of clusters having IC at least as great as the cluster under study.
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All three gene clusters are currently under study in our laboratory, where PCR amplification and Sanger sequencing have confirmed that the assembly and sequence reported here is correct.
Nevertheless, three groups of homogeneous care trajectories could be identified: (1) pregnant women with relatively high care consumption (cluster 1; 11 % of the population under study), (2) women with no care (cluster 2; 21 %), and (3) women with an intermediate level of care (cluster 3; 66 %).
Among the 6178 genes under study, 4874 were clustered into 30 clusters.
It is worth to mention that the great majority of the sequences from the soil samples under study were clustered separately from reference sequences, showing to be more closely related to each other.
To investigate the influence of poor quality genotyping on the performance of the super-locus method we excluded any SNPs considered to be of low quality, based on clustering, from the gene region under study and re-analysed.
Figure 1 depicts the complete linkage clustering of 69 archaeal organisms under study on the basis of the average frequencies of occurrences of different amino acid residues in the respective proteomes (for organism details, see Additional file 1).
This cluster was missed in other genomes under study.
By applying the second criterion we hypothesized that a small number of control sequences in the subtree is more likely to reflect a transmission cluster (possibly originating from the patient under study) than a genuine case of superinfection.
Cluster analysis of the real-time PCR results of the genes under study revealed two distinct clusters in the primary tumour samples.
In addition, the phylogenetic tree built from all EUL domains under study, revealed distinct clusters.
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