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Each molecule then grows into a single-molecule cluster sequencing template through in situ amplification.
Each molecule grows into a single-molecule cluster sequencing template through Situ amplification.
Each molecule grows into a single-molecule cluster sequencing template through in situ amplification.
Each molecule was then allowed to grow into a single-molecule cluster sequencing template through in situ amplification.
After denaturation, the single-chain fragments were fixed onto the Solexa Sequencing Chip (Flowcell) and subsequently grown into single-molecule cluster sequencing templates via in situ amplification on an Illumina Cluster Station.
The obtained cDNA library had an average size of 150-300 bp (the obtained length was actually shorter; see Results) and were used in ligation of adaptors for cluster sequencing.
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Within these two large clusters, sequencing technology choice contributed additional, but minor, divergences (r-values 0.8 0.9).
The low G + C sequences also include some Proteobacteria (1361 sequences), cluster II sequences (330 sequences) and cluster IV sequences (254 sequences).
To select a non-redundant set, we used mkvtree in the Vmatch suite (see URLs) to index the sequences by length; Vmatch was used to cluster sequences, including clusters with size of 1 (singlets).
Casein phosphopeptides (CPP) contain the cluster sequence of -Ser (P -Ser (P -Ser (P -Ser-Glu from casein [33, 34].
Then, for each cluster sequence, we also train a three-state right-left HMM model for the audio data.
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CEO of Professional Science Editing for Scientists @ prosciediting.com