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In each cluster, proteins with dense communications are considered as the core and proteins containing less communication are removed as noises.
We also use electrostatic similarity indices to cluster proteins with common electrostatic properties.
This is illustrated in Figure 2. Promiscuous domains present a significant challenge to orthology prediction: all methods of orthology prediction that are based either implicitly (as in graph-based methods) or explicitly (as in domain-based phylogenetic methods) on local alignment can incorrectly cluster proteins with different domain architectures into orthology groups.
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Furthermore, within the same species, it appears that there are two types of zinc cluster proteins, some with strong homology to CZT-1 and others with weak homology.
Using a 'metal-first' approach, we computationally designed, prepared, and characterized a four-iron four-sulfur (Fe4S4) cluster protein with a non-natural α-helical coiled-coil fold.
The single gene that had neither a Pho4 binding site nor a GGGAGG motif, EDS1, encodes a zinc cluster protein with unknown function.
The last cluster, cluster 12 shows proteins with a relatively similar expression level in all protein isolates.
On analyzing the phylogenetic profiles of T6SS proteins (Table S2), three significant protein clusters (proteins with similar profiles) were obtained.
Besides the clustered proteins with known functions, many families of hypothetical proteins and unique proteins (ORFans and proteins with only distant homologs in non-LAB) were found.
Alternatively, we tested for the presence of 3,560 strongly homologous groups, identified by clustering proteins with higher than 50% amino acid similarity.
Some toxin clusters have associated HA proteins with hemagglutinating properties, while others lack these proteins.
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