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Five picomoles of DNA from each strain were loaded onto two lanes of the sequencing chip, and the clusters were generated on the cluster generation station of the GAIIx using the Illumina cluster generation kit.
Clusters were generated in a cBot Cluster Generation System using the Paired-End Cluster Generation Kit v2-HS and sequenced on the Illumina HiSeq 2000 platform (Illumina) with a 2x50 base-pairs (BP) paired-end mode.
The automated cBot Cluster Generation System (Illumina) was used to generate clusters on the flow cell.
Clusters were generated using Illumina's Single Read Cluster Generation Kit v4 (GD-103-4001) or Paired End Cluster Generation Kit v4 (PE-203-4001), respectively.
Standard Illumina chemistry was used for cluster generation.
Cluster generation of the prepared samples was performed using a HiSeq Paired-End Cluster Generation kit according to manufacturers instructions.
Data from our analysis elucidated an order within the cluster generation.
Cluster amplification was performed using a Paired-End Cluster Generation Kit v2.
Using an Illumina Cluster Station, the diluted libraries were hybridized to an 8-lane flow cell, followed by cluster generation by isothermal amplification using a DGE-Small RNA Cluster Generation Kit v1.0 (Illumina, San Diego, CA).
The PCR products at 85 bp DNA band was excised and purified for cluster generation and sequencing analysis.
The library was sequenced on Illumina GA sequencing instrument according to standard Illumina cluster generation and sequencing protocols.
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