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Cluster figures were made using the MultiExperiment Viewer (MeV) in the TM4 suite of software tools (www.tm4.org) (Saeed et al., 2006).
Interestingly, 13 clusters (<92% similarity clustering in Cd-hit) of comparable relative abundance (∼0.4% of sample for each cluster, Figures 2 and S2) also clustered with C. watsonii.
In fact, overexpression of miR-17-5p, but not of miR-92, increased the number of colonies formed by SK-N-AS in a semisolid medium, as observed for cells overexpressing the entire cluster (Figures 2D and 3D).
Furthermore, genes encoding key enzymes in the poly-unsaturated fatty acid (PUFA) pathway (FADS1, FADS2 and ELOVL5), and an acyltransferase (AGPAT3) with a key role in the phospholipid pathway, were identified in the same gene cluster (Figures 5A and B).
Genes encoding digestive enzymes produced by acinar cells such as amylase (AMY2A), elastase (ELA3A, ELA3B), carboxypeptidase A (CPA2), pancreatic lipase (PNLIP), and trypsin (PRSS2) were enriched in this cluster (Figures 1, "Acinar" cluster).
The crystalline cluster is located near the sulcus (a groove where the longitudinal flagellum resides) (Figure 2e: arrows), and the cluster architecture was more or less the same in all cells: the rows followed the curvature of the sulcal groove (Figures 3a, b) with the flagellar basal body lying close to the cluster (Figures 3f, g: arrows).
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This observation is supported by a cross-sectional view of the cluster (Figure 3a).
All C. sakazakii strains formed one cluster (Figure 1).
Further microRNA clusters found in human neutrophils were the let7a-1 and let7a-3 clusters (Figure 3e), the miR-29a and miR-29c clusters (Figure 3f), and the miR-181a cluster (Figure 3g).
CXCR4- and dual-tropic sequences preferentially occupy the outer regions of the cluster (Figure 4).
The resulting protein differs in the first zinc finger cluster (Figure 6C).
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