Clustering indicates a clear demarcation between normal and most cancer samples (Figure 5).
Briefly, sections were scored numerically between 0 and 12 on the basis of presence of cell clusters, fissures, loss of demarcation and haematoxophilia (indicating reduced proteoglycan content).
In brief, sections were scored for the presence of cell clusters, fissures, loss of demarcation and haematoxophilia (indicating reduced proteoglycan content): a score of 0 to 3 indicates a histologically normal (non-degenerate) IVD and a grade of 5 to 12 indicates evidence of degeneration.
Sections were scored numerically between 0 and 12 based on the presence of cell clusters, fissures, loss of demarcation and haematoxophilia (indicating reduced proteoglycan content); a score of 0 to 3 indicates a histologically normal (nondegenerate) IVD and a grade ≥4 indicates evidence of degeneration, as described previously [ 18, 35].
Sections were scored numerically between 0 and 12 based on the presence of cell clusters, fissures, loss of demarcation and haematoxophilia (indicating reduced proteoglycan content); a score of 0 to 3 indicates a histologically normal (non-degenerate) IVD and a grade ≥4 indicates evidence of degeneration, as described previously [ 15].
The regional NPopC staff directed the mapping of the surveillance area and its demarcation into smaller units (clusters) for easy enumeration, by re-demarcating the current Enumeration Area (EA) census maps from the NPopC into smaller units referred to as clusters.
On the other hand, a sharp demarcation was readily apparent between clusters of transplanted nodular hepatocytes and the positive immunostaining for MMP-2 (Fig. 5d f).
In brief, sections were scored for the presence of cell clusters and fissures and for loss of demarcation and haematoxophilia (indicating reduced proteoglycan content).
Panmunjom is home to a cluster of blue huts that stand directly on the military demarcation line, and where officials from North Korea and the US-led UN command meet for negotiations.
While it is possible that transcript profiling of a higher number of DSD-patients could lead to demarcation of even diagnosis-specific patient clusters, the consistency of the transcript profiles across the different diagnoses argues strongly that these four categories are relatively robust.
