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The dominating cluster assemblies of Gallibacterium spp. were compared to the assemblies of fimbriae found in E. coli.
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Iba57 forms a complex with the scaffold protein IscA (Isa1p and Isa2p in yeast), which plays a specific role in [4Fe4S] cluster assembly of aconitase-type proteins and the functional activation of mitochondrial radical-SAM FeS proteins [39].
In vivo cluster assembly of GST FNR fusion proteins was promoted under anaerobic conditions, as described previously [ 25].
Mutations of residues D4 or G17 G21 had only mild effects on Fe-S cluster assembly of Rli1.
When we analyzed the de novo Fe-S cluster assembly of Rli1 under anaerobic conditions, depletion of Yae1 or Lto1 led to similar Fe-S cluster assembly defects as depletion of Nbp35.
The decreased Fe-S cluster assembly of Rli1 observed for this Lto1 W → A mutation is therefore best explained by the weakened contact of Lto1 to the CIA machinery thus limiting the recruitment of the Rli1 client.
Depletion of Yae1 by blue light exposure diminished de novo Fe-S cluster assembly of Rli1, yet did not affect the stability of the clusters, clearly documenting that Yae1 (and presumably Lto1) function as maturation rather than stabilization factors for Rli1.
Toward this aim, we selected 27,513 of the cDNA clones by four successive clustering assemblies of the soybean cDNAs performed as the 5' EST data accumulated.
Structural annotation can also be performed by the CA. The submitted model can be visualized alongside the Osa1 model(s), Rice Transcript Assemblies (which are clustered assemblies of ESTs, full length cDNAs, and mRNAs [ 29]) as well as full-length cDNAs aligning in the same region.
It consists of a low-density cluster-assembly of carbon atoms strung together in a loose three-dimensional web, in which the atoms are bonded trigonally in six- and seven-membered rings.
The bacterial-type FeS cluster assembly genes of E. histolytica suggest their lateral acquisition from epsilon proteobacteria.
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