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Using the Clover structure, we rationally engineered a non-photoactivatable redox sensor, roClover1, and determined its structure as well as that of its parental template, roClover0.1.
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In the analysis of RNA modification in tRNAs and miRNAs, we had to determine the clover leaf structure of tRNAs and fold-back structures of miRNAs.
One of the tRNAs (tRNA-Tyr GTA) has a two stem clover leaf structure.
Analysis of the four homoeologous BAC pairs through sequencing in this study provide an insight into white clover genome structure.
In the perennial ryegrass mitochondrial genome, twenty-four tRNAs display a classical clover leaf structure, whereas each of the two tRNA-Ser (tRNA-SerTGA and tRNA-SerGCT) fold into an unusual four-loop secondary structure.
This feature is shared with the C. raphaelis mtDNA [ 6] but is not a general feature of lepidopteran mtDNA as proved by A. honmai that has all tRNAs with a complete clover leaf structure [ 7].
Finally, RNA editing in trnM is required for the formation of a standard clover-leaf structure.
For instance, RNA editing in trnM is required for the formation of a standard clover-leaf structure.
In addition, after being edited, despite no difference in free energy change (ΔG), the clover-leaf structure was restored as predicted by tRNAscan-SE 1.21 (Fig. 2b), which implied the functional importance of RNA editing for the trnM.
Most of the tRNAs are capable of forming the typical clover-leaf structure, with the exception of tRNAAla (which lacks both the DHU and TψC arms), tRNASer AGN), tRNATyr and tRNAVal (which lacks only the DHU arm).
Both the tRNAGln genes can assume stable clover-leaf structures.
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