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To correct for this, the assembly was artificially broken into three contigs at these regions and was rejoined using the gap closure software PBJelly [ 53].
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Several strategies proposed and implemented for improving genome assemblies include the use of varying size fragment libraries, longer length reads, gap-closure software and postprocessing to detect misassemblies (Treangen and Salzberg, 2012).
Forced closure of software products, as they say, is the mother of invention.
The group in charge of the attraction said that it would use the closure to update "software and hardware" related to managing visitors, Xinhua said.
While PeopleSoft blamed its earnings shortfall on the bad publicity caused by the hostile takeover bid launched by rival Oracle, Morgan Stanley sees at least two other contributing factors: the failure of two large deals it was tracking to close and a "general softness in closure rates in software at the end of June".
These results were found to be statistically significant by quantitative analysis of wound closure using Scion image software (P<0.01).
Gap closure was quantified (Tscratch software) at both 0 and 24 hours and percent of closure determined with the following equation: percent of closure = average of ((gap area: 0 hour) – (gap area: 24 hours))/(gap area: 0 hour) using images from 12 different microscopic fields per well (4X magnification).
Wound closure was calculated using Spot software.
Microscopic imaging of wound closure was analysed using CellD software [ 24].
The relative area of wound closure was measured using ImageJ software [ 35] (NIH Image, Bethesda, MD, USA).
The area of wound closure was quantified using ImageJ software, and the relative inhibition of migration was determined.
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