Your English writing platform
Discover LudwigExact(10)
It catalyses a multistep ring closure reaction yielding PNP and inorganic phosphate (Pi).
An efficient one-pot strategy for the synthesis of a new family of imidazo[1,4]diazepines has been developed and its mechanism has been proposed, which follows a seven-membered ring closure reaction.
The steady-state scans shown in Figure 4A were taken after the closure reaction was complete.
No closed state accumulates if Mg2+ is included in the closure reaction.
Two FRET constructs were designed to probe distinct aspects of the closure reaction, relying on a Cys-free version of hTRAP1 (Lavery et al., 2014).
This combination allowed for sufficient light intensity for triggering the ring closure reaction while still enabling imaging of the fluorescence of the open form.
Similar(50)
Caution is obviously needed before making broad generalizations about CMe2 groups accelerating ring closure reactions!
After forming heterodimers, closure reactions were initiated with AMPPNP over a temperature range mirroring our SAXS experiments and the change in FRET was monitored.
We were able to decouple the closure and hydrolysis steps by removing MgCl2 from the closure reactions in the presence of nucleotide and find that closure is much slower than hydrolysis, with both WT and Δstrap hTRAP1.
It happens since in less polar solvents the ring-closure reaction proceeds easier, since the exited state of OF is stabilized in planar conformation close to the structure of CF, opposed to twisted form in polar solvents [18, 19].
Pyridoxine 5′-phosphate (PNP) synthase is the last enzyme in the de novo biosynthesis of vitamin B6 catalyzing the complicated ring-closure reaction between 1-deoxy-d-xylulose-5-phosphate and 1-amino-acetone-3-phosphate.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com