Beach closures due to unsafe levels of domoic acid in shellfish tissues from the harmful algal bloom Pseudo-nitzschia species are relatively recent phenomena in Puget Sound (WA), with the first closure documented in 2003 [ 56].
Possible complications such as avascular necrosis, nonunion, proximal synostosis, heterotopic ossification, infection, and premature physeal closure were documented.
Released in 1997, the Closure video documented highlights from the tour, including full live videos of "Eraser", "Hurt" and a one-take "March of the Pigs" clip made for MTV.
The closure was documented over a period of 12 h as shown in Figure 5A.
Over a period of approximately 21 days, wound closure was documented in 1,575 (47.1%) patients with a mean duration to closure of 11.2 ± 7.3 days (median 10.0 days).
Previous detailed analysis of spinal neural tube closure has documented three 'modes' of closure, characterized by the precise morphology of the neural folds (6).
No final closure was documented for two patients (D131 and D269), one patient (D186), and five patients (D90, D142, D143, D143, D176) in Groups I, II, and III respectively.
Cells were treated with vehicle or IC20 ACA (10.0 μM) in serum-free medium for 24 h and microscopic images describing speed of wound closure was documented at various time intervals using an inverted fluorescence microscope, Nikon Eclipse TS-100 and analyzed using TScratch software, Version 1.0 (MathWorks Inc).
Subsequent closures were documented in 2005 [ 57], suggesting that domoic acid may continue to threaten shellfish harvest areas in the future.
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