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No significant KEGG pathway enrichment was observed in young mice after full wound closure (Day 21).
Main outcome measures Estimated RBC folate concentration at time of neural tube closure (day 28 of gestation) and risk of neural tube defects.
The enrichment analysis revealed that in aged WT mice, in spite of the histologically confirmed full wound closure (Day 21), numerous pathways are still significantly activated as compared to the intact skin (Table 1, Suppl. Table 2).
Shifting the temporal window so that it starts just following neural tube closure (day 26) yielded the largest fourth nonzero quartile OR = 7.6 [95% confidence interval (CI), 3.1 18.6].
For R0 = 1.2, the peak incidence is about 140,000 with 1 week closure; 134,000 with 2 weeks; 120,000 with 4 weeks; 94,500 with 8 weeks; and 12,000 with 16 weeks; the peak day is later for 1, 2, 4, and 8 weeks of closure (day 52, 56, 76, and 112, respectively) but earlier for 16 weeks of closure (day 32).
We developed estimates for the distribution of red blood cell folate concentration at neural tube closure (day 28 of gestation) among the 228 456 Community Intervention Project study participants by using the posterior estimates of the parameters of the concentration model (supplementary table D).
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