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The protocol involved dispensing 100 μL of water (or another buffer/solvent) onto the patch, incubating for up to 10 min to extract the model analyte (sulforhodamine), closing the cap and centrifuging for 20 s at 300 g to collect the extracted analyte solution.
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We hope that with the publication of the methodology of our study, we will contribute to discussion on how studies on naturalistic treatments and concordance of treatment guidelines can be designed, helping to close the cap between evidence based treatments in guidelines and care in every day clinical practice.
You have to close the cap of container tightly.
Empty it of water and securely close the cap.
Put them directly into the bottle and close the cap tightly.
Drop an Alka Seltzer in, close the cap so it clicks.
If you're using a bottle with a cap, you can close the cap and shake it.
Make sure to close the cap of the water bottle after spitting into it, however, as it could spill.
If not, close the cap and refrigerate the pop until it feels cold enough to enjoy as usual.
Let the Glue stick sit horizontally on the newspaper to let the ink flow into the bottom and close the cap.
Pour the salt water into the container so that the water will immerse the nail once you close the cap and hammer it in.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com