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Furthermore, ABA-induced stomatal closing responses in these complementation lines confirmed the need to mutate both the S59 and S120 sites to alanine to significantly impair ABA-induced stomatal closing in planta.
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More on this in my closing response to Bapteste.
The presented combined genetic, cell signaling and physiological response analyses provide strong evidence for a concomitant requirement of both the Ca2+-dependent and Ca2+-independent branches to trigger a robust (Hetherington, 2001) downstream stomatal closing response.
To address the context we assessed Telford's [ 46] principles of successful involvement in National Health Service research (Table 1) by seeking agreement with eight closed response options, and also three closed response options about participation in the project (Table 1).
Closed response survey data were entered into SPSS© version 19 (SPSS IBM Corporation, Armonk, NY, USA).
Those who answered yes were asked further closed response questions regarding frequency (once, 2 5 times.
This included primarily closed response questions; response option questions, Likert scales and the inclusion of a free text 'other' category (Additional file 1).
Details of the baseline questionnaire are provided in Additional file 2. The questionnaire contained mostly closed response questions (and a small number of open response questions).
Under these headings, participants were requested to use a combination of closed response tick boxes and free text to record their responses.
The questionnaire was adapted from a similar survey used in the UK[ 14] and included open ended and closed response questions.
Information regarding students' age, gender, school year and ethnic/cultural background (Table 1) was provided by means of a maternal telephone interview as well as a self report questionnaire completed by each student with closed response questions.
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