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To test this notion, they compared genes in flies and crustaceans, which are insects' closest relatives with gills.
In our initial BLAST search through GenBank using DNA sequences of the three new species, the closest relatives with the highest hit scores were species in the recently recircumscribed Primulina as expected based on morphological observations.
Pericles, following Athenian custom, was first married to one of his closest relatives, with whom he had two sons, Paralus and Xanthippus, but around 445 BC, Pericles divorced his wife.
All sequences were further analysed by BLAST algorithm [87] against sequences held in the GenBank database (http://www.ncbi.nlm.nih.gov/BLAST/) and the closest relatives with percent homology are used to report taxonomic affiliations to the family or group level.
The closest relatives with respect to their DNA-binding domain were ZNF7 and ZNF251.
As expected, these results confirm that artiodactyls and carnivores are the closest relatives, with primates being the most distant.
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Additional identification using MALDI TOF MS analysis also showed coherent identification of P. putida as the closest relative with matching score constantly above the values of 1.9.
Banksia ilicifolia and B. oligantha form a clade, that is they are each other's closest relative, with Banksia cuneata resolving as the next closest relative, suggesting a monophyletic B. subg.
Surprisingly, raphines are not the most primitive columbid, instead they are grouped with the Nicobar pigeon as their closest relative, with other closely related birds being the crowned pigeons and tooth-billed pigeon.
For example, Enterobacter gergoviae was selected to compare results obtained with the tiled sequence of E. coli, its closest relative, with results obtained after hybridization on the RAS of the Citrobacter/Escherichia/Salmonella/Enterobacter (CESE) ancestor.
To address this question, we compared the conservation of orthologous genes between N. crassa and the closest relative with a sequenced genome, Chaetomium globosum, a member of the Sordariomycetes within the Pezizomycotina clade.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com