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It is also important to monitor the process closely to derive lessons learnt.
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Reconstructed ancestral sequences (RAS) have the desirable property of being more closely related to derived sequences than the latter are among themselves.
This was confirmed by flow cytometric analysis which showed the presence of double positive Gr-1 (myeloid differentiation antigen) and CD11b (macrophage and granulocyte marker) cell populations (71%) which compared closely to those derived from wt-iPSCs (85%) (Figure 3C).
Despite huge variation in ranges, both AD and PDD median estimates correspond closely to those derived from Northern Europe.
Effect estimates from indirect comparisons matched closely to estimates derived from pairwise comparisons.
To derive closely paired in vitro data from these litters of ΔF-CFTR mice, tracheae were excised from these same mice in which CFTR NPD measurements were performed previously to isolate and establish mouse tracheal epithelial (MTE) cell monolayers grown on permeable filter supports in primary culture.
These estimates closely matched the quantities used to derive the data of 0.42 and 0.48, respectively.
In the presence of formate, M. stadtmanae has been shown to derive C8 from C2 of acetate (most consistent with the use of a tetrahydrofolate C1 carrier), while closely related M. smithii does not derive C8 from acetate [ 32].
The effect estimates from both the fixed and random-effects models were comparable and matched closely to the estimates derived from the pairwise comparisons in both direction and magnitude.
Finally, the predicted secondary structure (i.e. α-helices, β-strands and coiled coils) of the Oculina protein corresponds closely to the experimentally derived X-ray structure of 1tl2.
On the other hand, if the research is tied closely to or directly derives from clinical practice, such an equal split may be realistic.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com